Hotspot

trnR-UCU-atpA

LSC · 10172-11165 · 994 bp

Back to Alniaria

Pi 0.0308
MarkerSeek score 57.00
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
994
variable_sites
24
pi
0.0308
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.64
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 AGCCTTCCAAGCTAACGATG ACAATCTCAATCATCCCCGC 50.0 50.0 58.0 58.0 1111-1568 bp 1.0 85.1
trnR-UCU-atpA_p2 CCTAGCCTTCCAAGCTAACG ACAATCTCAATCATCCCCGC 55.0 50.0 58.1 58.0 1114-1571 bp 1.0 84.9
trnR-UCU-atpA_p3 AGCCTTCCAAGCTAACGATG TCAGACCGGCCATTAATGTG 50.0 50.0 58.0 58.0 1316-1773 bp 1.0 84.8
trnR-UCU-atpA_p4 AGCCTTCCAAGCTAACGATG TTCGGTTTCCAGGGTAGGAT 50.0 50.0 58.0 58.0 1291-1748 bp 1.0 84.8
trnR-UCU-atpA_p5 CCTAGCCTTCCAAGCTAACG TCAGACCGGCCATTAATGTG 55.0 50.0 58.1 58.0 1319-1776 bp 1.0 84.6