Hotspot

trnH-GUG-psbA

LSC · 102-362 · 261 bp

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Pi 0.0243
MarkerSeek score 67.70
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
261
variable_sites
9
pi
0.0243
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 ATATTATGGGCGAACGACGG GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 438-442 bp 1.0 80.8
trnH-GUG-psbA_p2 ATATTATGGGCGAACGACGG CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 388-392 bp 1.0 80.7
trnH-GUG-psbA_p3 ATATTATGGGCGAACGACGG TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 391-395 bp 1.0 80.6
trnH-GUG-psbA_p4 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 377-381 bp 1.0 80.6
trnH-GUG-psbA_p5 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 391-395 bp 1.0 80.5