Hotspot

ccsA-ndhD

SSC · 109791-109996 · 206 bp

Back to Alloteropsis

Pi 0.0829

MarkerSeek score 73.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 206
variable_sites: 25
pi: 0.0829
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGTATCAATCTGTTAGGAATAGGT	GTTGACAGGGTAGAGGCTCT	36.0	55.0	57.1	58.4	314-315 bp	1.0	47.1
ccsA-ndhD_p2	TGGTATCAATCTGTTAGGAATAGGT	CTCGCTATCTGTTGACAGGG	36.0	55.0	57.1	57.8	324-325 bp	1.0	46.9
ccsA-ndhD_p3	TGGTATCAATCTGTTAGGAATAGGT	TCGCTATCTGTTGACAGGGT	36.0	50.0	57.1	58.4	323-324 bp	1.0	46.9
ccsA-ndhD_p4	TGGTATCAATCTGTTAGGAATAGGTT	CTCGCTATCTGTTGACAGGG	34.6	55.0	57.7	57.8	324-325 bp	1.0	46.9
ccsA-ndhD_p5	TTGGTATCAATCTGTTAGGAATAGGT	CTCGCTATCTGTTGACAGGG	34.6	55.0	57.7	57.8	325-326 bp	1.0	46.9