Hotspot

ndhF-rpl32

SSC · 107025-107950 · 926 bp

Back to Alloteropsis

Pi 0.0391

MarkerSeek score 71.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 926
variable_sites: 48
pi: 0.0391
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GAAGAGGGAATTGTGCGGAT	TCGTTGCGGCTAGAAAATGA	50.0	45.0	58.0	57.9	1701-1799 bp	1.0	86.0
ndhF-rpl32_p2	ACACATTCCGACAAGTTCCC	TCGTTGCGGCTAGAAAATGA	50.0	45.0	58.1	57.9	1433-1531 bp	1.0	85.9
ndhF-rpl32_p3	CACATTCCGACAAGTTCCCA	TCGTTGCGGCTAGAAAATGA	50.0	45.0	58.1	57.9	1432-1530 bp	1.0	85.8
ndhF-rpl32_p4	GGAATTGTGCGGATTTTGCA	TCGTTGCGGCTAGAAAATGA	45.0	45.0	57.9	57.9	1695-1793 bp	1.0	85.7
ndhF-rpl32_p5	GAAGAGGGAATTGTGCGGAT	AATTTCACCATGGCGGCTAA	50.0	45.0	58.0	58.2	2288-2396 bp	1.0	85.3