Hotspot

rpoA-rps11

LSC · 76940-77003 · 64 bp

Back to Alloteropsis

Pi 0.0521

MarkerSeek score 70.60

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 5
pi: 0.0521
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	GCAAGGCAATACCGACAGTA	GCTATTCGTACGGTGGGTTT	50.0	50.0	58.0	58.0	386 bp	1.0	82.6
rpoA-rps11_p2	TCCGCGATCTCTCTTGATCT	GCTATTCGTACGGTGGGTTT	50.0	50.0	58.0	58.0	706 bp	1.0	82.3
rpoA-rps11_p3	GTGTTTGGGTGGATCCTGTT	GCTATTCGTACGGTGGGTTT	50.0	50.0	58.0	58.0	275 bp	0.666667	69.1
rpoA-rps11_p4	GTGTTTGGGTGGATCCTGTT	AAAGTGCGTATTGGTTTGCG	50.0	45.0	58.0	58.0	497 bp	0.666667	68.4
rpoA-rps11_p5	GTGTACTCTTCCTCTGCGTG	GCTATTCGTACGGTGGGTTT	55.0	50.0	58.0	58.0	948 bp	0.333333	53.1