Hotspot

psbT-psbN

LSC · 72197-72252 · 56 bp

Back to Alloteropsis

Pi 0.0705

MarkerSeek score 86.30

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 56
variable_sites: 5
pi: 0.0705
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TTCCGAGAACCACCTAAGGT	CCATTCGAAGAACATGGGGA	50.0	50.0	58.0	57.6	112-116 bp	1.0	82.8
psbT-psbN_p2	TTCCGAGAACCACCTAAGGT	TCCATTCGAAGAACATGGGG	50.0	50.0	58.0	57.6	113-117 bp	1.0	82.8
psbT-psbN_p3	TCTTCTTCCGAGAACCACCT	CCATTCGAAGAACATGGGGA	50.0	50.0	58.0	57.6	117-121 bp	1.0	82.6
psbT-psbN_p4	TCTTCTTCCGAGAACCACCT	TCCATTCGAAGAACATGGGG	50.0	50.0	58.0	57.6	118-122 bp	1.0	82.6
psbT-psbN_p5	TCTTCTTCCGAGAACCACCT	TATATACCGCGTTTGGGCAA	50.0	45.0	58.0	57.4	158-162 bp	1.0	79.5