Hotspot

petG-trnW-CCA

LSC · 65457-65575 · 119 bp

Back to Alloteropsis

Pi 0.0672

MarkerSeek score 74.10

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 119
variable_sites: 12
pi: 0.0672
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	GGGATTATTCGTGACTGCGT	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	216-221 bp	1.0	83.5
petG-trnW-CCA_p2	GGGAATATTTGCCCGTTTGC	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	867-876 bp	1.0	82.4
petG-trnW-CCA_p3	CCGGCTAGACTCTTTTCCAC	CAAAACCCGATGTCGTAGGT	55.0	50.0	58.0	57.9	397 bp	0.333333	52.5
petG-trnW-CCA_p4	CCGGCTAGACTCTTTTCCAC	AAACCTGCTAAAGCCCCAAA	55.0	45.0	58.0	57.9	1097 bp	0.333333	52.5
petG-trnW-CCA_p5	CCGGCTAGACTCTTTTCCAC	CTTGGTAGCGCGTTTGTTTT	55.0	45.0	58.0	57.9	621 bp	0.333333	52.3