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psbE-petL

LSC · 63917-65064 · 1148 bp

Back to Alloteropsis

Pi 0.0213

MarkerSeek score 68.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1148
variable_sites: 36
pi: 0.0213
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.0	58.5	1306-1358 bp	1.0	79.2
psbE-petL_p2	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	45.0	50.0	58.0	58.5	1304-1356 bp	1.0	79.2
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1295-1347 bp	1.0	79.0
psbE-petL_p4	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1293-1345 bp	1.0	78.9
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	58.2	58.5	1297-1349 bp	1.0	77.5