Hotspot

rbcL-psaI

LSC · 58062-58977 · 916 bp

Back to Alloteropsis

Pi 0.0277

MarkerSeek score 63.80

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 916
variable_sites: 38
pi: 0.0277
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GGCTTCAGGGGGTATTCATG	CCAAGATAACTGGAAGCCCC	55.0	55.0	58.0	57.9	1819-1870 bp	1.0	82.5
rbcL-psaI_p2	GGCTTCAGGGGGTATTCATG	ACATTACGACTCCCTGTGGA	55.0	50.0	58.0	58.1	1884-1935 bp	1.0	82.4
rbcL-psaI_p3	GATCATATCCACGCCGGTAC	CCAAGATAACTGGAAGCCCC	55.0	55.0	58.0	57.9	1980-2031 bp	1.0	82.3
rbcL-psaI_p4	ATATCCACGCCGGTACAGTA	CCAAGATAACTGGAAGCCCC	50.0	55.0	58.0	57.9	1976-2027 bp	1.0	82.3
rbcL-psaI_p5	GATCATATCCACGCCGGTAC	ACATTACGACTCCCTGTGGA	55.0	50.0	58.0	58.1	2045-2096 bp	1.0	82.3