Hotspot

trnT-TGT-trnF-GAA

LSC · 47804-49520 · 1717 bp

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Pi 0.0263
MarkerSeek score 66.80
Primer available yes
Label trnT-TGT-trnF-GAA

Feature Metadata

label_name
trnT-TGT-trnF-GAA
feature_type
igs
region
LSC
length_bp
1717
variable_sites
67
pi
0.0263
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-TGT-trnF-GAA_p1 ATTTGAACCGATGACCCTCG TTTGAACTGGTGACACGAGG 50.0 50.0 58.0 58.1 1831-1840 bp 1.0 83.2
trnT-TGT-trnF-GAA_p2 ATTTGAACCGATGACCCTCG CTGTGCCAGGAACCAGATTT 50.0 50.0 58.0 58.1 1848-1857 bp 1.0 82.9
trnT-TGT-trnF-GAA_p3 CGATGACCCTCGCATTACAA TTTGAACTGGTGACACGAGG 50.0 50.0 58.1 58.1 1823-1832 bp 1.0 82.9
trnT-TGT-trnF-GAA_p4 ATTTGAACCGATGACCCTCG GTGCCAGGAACCAGATTTGA 50.0 50.0 58.0 58.1 1846-1855 bp 1.0 82.8
trnT-TGT-trnF-GAA_p5 CGATGACCCTCGCATTACAA CTGTGCCAGGAACCAGATTT 50.0 50.0 58.1 58.1 1840-1849 bp 1.0 82.6