Hotspot

trnR-TCT-trnfM-CAT

LSC · 38036-38110 · 75 bp

Back to Alloteropsis

Pi 0.0516
MarkerSeek score 73.70
Primer available yes
Label trnR-TCT-trnfM-CAT

Feature Metadata

label_name
trnR-TCT-trnfM-CAT
feature_type
igs
region
LSC
length_bp
75
variable_sites
5
pi
0.0516
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-TCT-trnfM-CAT_p1 AGAAGACCTCTGTCCTATCCA ACTACCCGCTCCATATTC 47.6 50.0 56.9 53.0 122-129 bp 1.0 47.2
trnR-TCT-trnfM-CAT_p2 AGAAGACCTCTGTCCTATCCAT ACTACCCGCTCCATATTC 45.5 50.0 57.4 53.0 122-129 bp 1.0 47.2
trnR-TCT-trnfM-CAT_p3 GGTTTAGAAGACCTCTGTCCT ACTACCCGCTCCATATTC 47.6 50.0 56.4 53.0 127-134 bp 1.0 47.1
trnR-TCT-trnfM-CAT_p4 AGGTTTAGAAGACCTCTGTCCT ACTACCCGCTCCATATTC 45.5 50.0 57.9 53.0 128-135 bp 1.0 47.0
trnR-TCT-trnfM-CAT_p5 AGGTTTAGAAGACCTCTGTCC ACTACCCGCTCCATATTC 47.6 50.0 56.4 53.0 128-135 bp 1.0 47.0