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atpI-atpH

LSC · 33264-34106 · 843 bp

Back to Alloteropsis

Pi 0.0283

MarkerSeek score 68.80

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 843
variable_sites: 32
pi: 0.0283
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	823-912 bp	1.0	81.1
atpI-atpH_p2	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.5	57.8	845-934 bp	1.0	81.0
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	ATAACGGAAGCAGCAGCAAT	50.0	45.0	57.5	58.2	830-919 bp	1.0	80.8
atpI-atpH_p4	TAGCCGCAGCCTATATAGGT	TCCAGCAGCAATAACGGAAG	50.0	50.0	57.5	58.3	840-929 bp	1.0	80.6
atpI-atpH_p5	ATTTTTGCAACGTTAGCCGC	AAGCAGCAGCAATTAGTGGA	45.0	45.0	58.6	57.8	836-925 bp	1.0	80.6