Hotspot

trnD-GTC-psbM

LSC · 17140-18207 · 1068 bp

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Pi 0.0301

MarkerSeek score 69.30

Primer available yes

Label trnD-GTC-psbM

Feature Metadata

label_name: trnD-GTC-psbM
feature_type: igs
region: LSC
length_bp: 1068
variable_sites: 47
pi: 0.0301
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GTC-psbM_p1	TTCAATTGGTCAGAGCACCG	TGCGAGAATATTGACTTCCA	50.0	40.0	58.5	54.5	1127-1153 bp	1.0	53.2
trnD-GTC-psbM_p2	TTCAATTGGTCAGAGCACCG	GGAACTAGAATGAACAATGCAGT	50.0	39.1	58.5	57.1	1159-1185 bp	1.0	50.2
trnD-GTC-psbM_p3	TTCAATTGGTCAGAGCACCG	ATGCGAGAATATTGACTTCCA	50.0	38.1	58.5	55.1	1128-1154 bp	1.0	50.2
trnD-GTC-psbM_p4	TTCAATTGGTCAGAGCACCG	TGCGAGAATATTGACTTCCAT	50.0	38.1	58.5	55.1	1127-1153 bp	1.0	50.2
trnD-GTC-psbM_p5	TTCAATTGGTCAGAGCACCG	AGAATGAACAATGCAGTAGC	50.0	40.0	58.5	54.0	1153-1179 bp	1.0	49.1