Hotspot

trnfM-CAT-trnT-GGT

LSC · 13142-15903 · 2762 bp

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Pi 0.0345

MarkerSeek score 70.60

Primer available yes

Label trnfM-CAT-trnT-GGT

Feature Metadata

label_name: trnfM-CAT-trnT-GGT
feature_type: igs
region: LSC
length_bp: 2762
variable_sites: 134
pi: 0.0345
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAT-trnT-GGT_p1	CCTCAAGGTTATGAGCCTCG	TGAACCGATGACTTATGCCT	55.0	45.0	57.8	56.6	2851-2924 bp	1.0	73.0
trnfM-CAT-trnT-GGT_p2	CCTCGTGAGCTACCAAACTG	TGAACCGATGACTTATGCCT	55.0	45.0	58.3	56.6	2836-2909 bp	1.0	72.6
trnfM-CAT-trnT-GGT_p3	CCTCAAGGTTATGAGCCTCG	CCGATGACTTATGCCTTACCA	55.0	47.6	57.8	57.5	2847-2920 bp	1.0	72.2
trnfM-CAT-trnT-GGT_p4	CCTCAAGGTTATGAGCCTCG	ACCGATGACTTATGCCTTACC	55.0	47.6	57.8	57.5	2848-2921 bp	1.0	72.2
trnfM-CAT-trnT-GGT_p5	CCTCGTGAGCTACCAAACTG	CCGATGACTTATGCCTTACCA	55.0	47.6	58.3	57.5	2832-2905 bp	1.0	71.8