Hotspot

ycf1

SSC · 125857-131382 · 5526 bp

Back to Allomorphia

Pi 0.00978

MarkerSeek score 46.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5526
variable_sites: 121
pi: 0.00978
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTTCGTTTAATTCAGATATTTGT	CCTTTTGGTTCTCCCCGAAA	25.0	50.0	52.4	58.0	164-170 bp	1.0	47.6
ycf1_p2	AGTTTCGTTTAATTCAGATATTTG	CCTTTTGGTTCTCCCCGAAA	25.0	50.0	52.2	58.0	165-171 bp	1.0	47.6
ycf1_p3	GTTTCGTTTAATTCAGATATTTGT	CCTTCCTTTTGGTTCTCCCC	25.0	55.0	52.4	58.1	168-174 bp	1.0	47.5
ycf1_p4	AGTTTCGTTTAATTCAGATATTTG	CCTTCCTTTTGGTTCTCCCC	25.0	55.0	52.2	58.1	169-175 bp	1.0	47.4
ycf1_p5	GTTTCGTTTAATTCAGATATTTGT	TTGGGGAATGGAAACTGACC	25.0	50.0	52.4	57.7	186-192 bp	1.0	46.8