Hotspot

ndhF

SSC · 112536-114773 · 2238 bp

Back to Allomorphia

Pi 0.0118

MarkerSeek score 52.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 59
pi: 0.0118
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TTTGAATGGTCCCTATCGCG	TCGATTGCACTATTAGCCTGA	50.0	42.9	58.1	56.9	2659-2671 bp	1.0	65.3
ndhF_p2	TGTCAACCTTCCGGGAAATG	TCGATTGCACTATTAGCCTGA	50.0	42.9	58.1	56.9	2909-2921 bp	1.0	64.8
ndhF_p3	CCTATCGCGGAAGGAGAAAC	TCGATTGCACTATTAGCCTGA	55.0	42.9	58.1	56.9	2648-2660 bp	1.0	64.5
ndhF_p4	TCGCGGAAGGAGAAACATTT	TCGATTGCACTATTAGCCTGA	45.0	42.9	57.8	56.9	2644-2656 bp	1.0	64.3
ndhF_p5	GTCAACCTTCCGGGAAATGA	TCGATTGCACTATTAGCCTGA	50.0	42.9	57.8	56.9	2908-2920 bp	1.0	64.0