Hotspot

ndhD-psaC

SSC · 119091-119233 · 143 bp

Back to Allomorphia

Pi 0.0396

MarkerSeek score 73.10

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 143
variable_sites: 10
pi: 0.0396
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	ACCCGCTAATATTGGTAAACCT	AAACAACTCGAAGCATGGGT	40.9	45.0	57.2	58.0	227-229 bp	1.0	61.8
ndhD-psaC_p2	ACCCGCTAATATTGGTAAACCT	GGCATGAAACAACTCGAAGC	40.9	50.0	57.2	58.0	233-235 bp	1.0	61.6
ndhD-psaC_p3	AACCCGCTAATATTGGTAAACCT	AAACAACTCGAAGCATGGGT	39.1	45.0	57.9	58.0	228-230 bp	1.0	59.1
ndhD-psaC_p4	AACCCGCTAATATTGGTAAACCT	GGCATGAAACAACTCGAAGC	39.1	50.0	57.9	58.0	234-236 bp	1.0	58.9
ndhD-psaC_p5	ACCCGCTAATATTGGTAAACCT	AGAGATGTGAATCCGCTTGC	40.9	50.0	57.2	58.3	287-289 bp	1.0	58.4