Hotspot

ndhF-rpl32

SSC · 114774-115681 · 908 bp

Back to Allomorphia

Pi 0.0263

MarkerSeek score 79.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 908
variable_sites: 49
pi: 0.0263
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGGAAGTGGAATGAGAGGT	TGAAAAAGCCTTCAATGCTGT	50.0	38.1	58.0	56.9	1038-1060 bp	1.0	70.5
ndhF-rpl32_p2	TGGGAAGTGGAATGAGAGGT	ATGAAAAAGCCTTCAATGCTGT	50.0	36.4	58.0	57.4	1039-1061 bp	1.0	66.5
ndhF-rpl32_p3	TGGGAAGTGGAATGAGAGGT	TCGCTAATGAAAAAGCCTTCA	50.0	38.1	58.0	56.2	1045-1067 bp	1.0	64.7
ndhF-rpl32_p4	TGGGAAGTGGAATGAGAGGT	TGAAAAAGCCTTCAATGCTG	50.0	40.0	58.0	55.1	1038-1060 bp	1.0	63.8
ndhF-rpl32_p5	TGGGAAGTGGAATGAGAGGT	GAAAAAGCCTTCAATGCTGT	50.0	40.0	58.0	55.0	1037-1059 bp	1.0	63.7