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rps8-rpl14

LSC · 81987-82269 · 283 bp

Back to Allomorphia

Pi 0.0223

MarkerSeek score 65.50

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 283
variable_sites: 14
pi: 0.0223
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ACGTCAGCAATAGTGTCTCT	CGATTGCCCGAGAATTGAGA	45.0	50.0	56.3	58.1	377-410 bp	1.0	70.4
rps8-rpl14_p2	ACGTCAGCAATAGTGTCTCT	TTGCCCGAGAATTGAGACAG	45.0	50.0	56.3	57.9	374-407 bp	1.0	70.2
rps8-rpl14_p3	ACGTCAGCAATAGTGTCTCT	GCCCGAGAATTGAGACAGTT	45.0	50.0	56.3	57.9	372-405 bp	1.0	70.2
rps8-rpl14_p4	GTCAGCAATAGTGTCTCTACCC	CGATTGCCCGAGAATTGAGA	50.0	50.0	58.0	58.1	375-408 bp	1.0	67.7
rps8-rpl14_p5	GTCAGCAATAGTGTCTCTACCC	TTGCCCGAGAATTGAGACAG	50.0	50.0	58.0	57.9	372-405 bp	1.0	67.4