Hotspot

rpl20-rps12

LSC · 70234-71069 · 836 bp

Back to Allomorphia

Pi 0.00634

MarkerSeek score 62.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 836
variable_sites: 13
pi: 0.00634
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTCGAATGAGCTCCACGAAA	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.0	57.8	980-1025 bp	1.0	81.9
rpl20-rps12_p2	TCGAATGAGCTCCACGAAAG	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.0	57.8	979-1024 bp	1.0	81.9
rpl20-rps12_p3	CACGAAAGCTTGACGCAAAT	ACAGCCAATCCGAAATGTCA	45.0	45.0	58.0	57.8	967-1012 bp	1.0	81.7
rpl20-rps12_p4	CTCGAATGAGCTCCACGAAA	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.0	57.7	985-1030 bp	1.0	81.3
rpl20-rps12_p5	TCGAATGAGCTCCACGAAAG	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.0	57.7	984-1029 bp	1.0	81.3