Hotspot

petL-petG

LSC · 66897-67088 · 192 bp

Back to Allomorphia

Pi 0.0391

MarkerSeek score 63.00

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 192
variable_sites: 16
pi: 0.0391
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TCATTTAGCCCCTTCATGCT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	56.9	58.0	621-628 bp	1.0	75.2
petL-petG_p2	TCATTTAGCCCCTTCATGCT	CAGAACGTGGGTCTCCAAAA	45.0	50.0	56.9	58.0	600-607 bp	1.0	75.2
petL-petG_p3	TCATTTAGCCCCTTCATGCT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	56.9	58.0	599-606 bp	1.0	75.0
petL-petG_p4	TCATTTAGCCCCTTCATGCT	AAGAAAGGGATGTAGCGCAG	45.0	50.0	56.9	58.0	891-897 bp	1.0	74.5
petL-petG_p5	TCATTTAGCCCCTTCATGCT	GCTCTTAGTTCAGTTCGGCA	45.0	50.0	56.9	57.9	618-625 bp	0.8	64.0