Hotspot

psbE-petL

LSC · 65656-66800 · 1145 bp

Back to Allomorphia

Pi 0.00909

MarkerSeek score 56.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1145
variable_sites: 24
pi: 0.00909
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CGGTAGGTACAGCTAGTCCA	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.0	58.0	2076-2098 bp	1.0	83.8
psbE-petL_p2	TCGTTCGGGTTTGATTGTGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	2168-2190 bp	1.0	83.6
psbE-petL_p3	TTTATCTTGCTTCCTCCGCC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	2421-2443 bp	1.0	83.3
psbE-petL_p4	CTTCCTCCGCCATTTCATCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	2412-2434 bp	0.8	74.5
psbE-petL_p5	TCCTCCGCCATTTCATCAAG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	2410-2432 bp	0.8	74.5