Hotspot

accD-psaI

LSC · 59819-60665 · 847 bp

Back to Allomorphia

Pi 0.0177

MarkerSeek score 60.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 847
variable_sites: 34
pi: 0.0177
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	ACAGTACCCGAGGATTCACA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	1034-1055 bp	0.8	76.2
accD-psaI_p2	TTGGAATGCTGGGCGATATC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	1123-1144 bp	0.8	75.5
accD-psaI_p3	CGATTGATCCAGGGACTTGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1567-1588 bp	0.8	75.0
accD-psaI_p4	ATTGATCCAGGGACTTGGGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1577-1586 bp	0.4	56.4
accD-psaI_p5	TTGATCCAGGGACTTGGGAT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1576-1585 bp	0.4	56.4