Hotspot

ndhK-ndhC

LSC · 51287-51346 · 60 bp

Back to Allomorphia

Pi 0.0367

MarkerSeek score 65.90

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 5
pi: 0.0367
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	TTTGGGTTGTTCGAGCAAGT	CATGGCGAAAAGGAGCATTG	45.0	50.0	58.2	58.1	129-135 bp	1.0	84.0
ndhK-ndhC_p2	TGGGTTGTTCGAGCAAGTAA	CATGGCGAAAAGGAGCATTG	45.0	50.0	57.4	58.1	127-133 bp	1.0	81.1
ndhK-ndhC_p3	TTGGGTTGTTCGAGCAAGTA	CATGGCGAAAAGGAGCATTG	45.0	50.0	57.4	58.1	128-134 bp	1.0	81.0
ndhK-ndhC_p4	TTTTGGGTTGTTCGAGCAAG	CATGGCGAAAAGGAGCATTG	45.0	50.0	57.1	58.1	130-136 bp	1.0	79.0
ndhK-ndhC_p5	TGGGTTGTTCGAGCAAGTAAA	CATGGCGAAAAGGAGCATTG	42.9	50.0	58.1	58.1	127-133 bp	1.0	77.4