Hotspot

trnC-GCA-petN

LSC · 28558-29403 · 846 bp

Back to Allomorphia

Pi 0.00875

MarkerSeek score 62.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 16
pi: 0.00875
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	919 bp	1.0	80.7
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CCTTTAGAGTCCACTTCGCC	50.0	55.0	58.1	58.0	978 bp	1.0	80.6
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CTTTAGAGTCCACTTCGCCC	50.0	55.0	58.1	58.0	977 bp	1.0	80.6
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	913 bp	1.0	79.2
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AGACGACCATTAAAGCAGCC	50.0	50.0	58.1	58.3	928 bp	1.0	78.6