Hotspot

atpH-atpI

LSC · 13803-14761 · 959 bp

Back to Allomorphia

Pi 0.0105

MarkerSeek score 66.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 959
variable_sites: 23
pi: 0.0105
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GAAGCAAGTCCGACAGCTAA	TAGCCGCCGCTTATATAGGA	50.0	50.0	57.9	57.9	997-1065 bp	1.0	84.0
atpH-atpI_p2	GAAGCAAGTCCGACAGCTAA	TTTTTGCAACTTTAGCCGCC	50.0	45.0	57.9	58.1	1009-1077 bp	1.0	84.0
atpH-atpI_p3	AACAGAAGCGGCAGAAATCA	TAGCCGCCGCTTATATAGGA	45.0	50.0	58.1	57.9	965-1033 bp	1.0	83.9
atpH-atpI_p4	AACAGAAGCGGCAGAAATCA	TTTTTGCAACTTTAGCCGCC	45.0	45.0	58.1	58.1	977-1045 bp	1.0	83.8
atpH-atpI_p5	CAGCAGCAATAACAGAAGCG	TAGCCGCCGCTTATATAGGA	50.0	50.0	57.8	57.9	975-1043 bp	1.0	83.4