Hotspot

atpF-atpH

LSC · 13044-13556 · 513 bp

Back to Allomorphia

Pi 0.0152

MarkerSeek score 63.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 513
variable_sites: 17
pi: 0.0152
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GGCCCAAGGAAAGGAAAGAA	GGATTGGTTGTCGCTTTAGC	50.0	50.0	58.0	57.5	591-598 bp	1.0	79.1
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GGATTGGTTGTCGCTTTAGC	50.0	50.0	57.9	57.5	623-630 bp	1.0	78.0
atpF-atpH_p3	TTGGCCCAAGGAAAGGAAAG	GGATTGGTTGTCGCTTTAGC	50.0	50.0	58.3	57.5	593-600 bp	1.0	76.8
atpF-atpH_p4	CATTGGCCCAAGGAAAGGAA	GGATTGGTTGTCGCTTTAGC	50.0	50.0	58.4	57.5	595-602 bp	1.0	76.3
atpF-atpH_p5	ATTGGCCCAAGGAAAGGAAA	GGATTGGTTGTCGCTTTAGC	45.0	50.0	57.6	57.5	594-601 bp	1.0	75.8