Hotspot

trnS-GCU-trnR-UCU

LSC · 7944-9731 · 1788 bp

Back to Allomorphia

Pi 0.0177
MarkerSeek score 59.40
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
1788
variable_sites
70
pi
0.0177
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 GCTTTAGTCCACTCAGCCAT AGAAGACCTATGTCCTATCCA 50.0 42.9 57.6 54.6 1850-1885 bp 1.0 45.4
trnS-GCU-trnR-UCU_p2 AGTCCACTCAGCCATCTCTC AGAAGACCTATGTCCTATCCA 55.0 42.9 58.5 54.6 1845-1880 bp 1.0 44.6
trnS-GCU-trnR-UCU_p3 GCTTTAGTCCACTCAGCCAT AGAAGACCTATGTCCTATCCAT 50.0 40.9 57.6 55.2 1850-1885 bp 1.0 43.9
trnS-GCU-trnR-UCU_p4 TAGTCCACTCAGCCATCTCT AGAAGACCTATGTCCTATCCA 50.0 42.9 57.2 54.6 1846-1881 bp 1.0 43.9
trnS-GCU-trnR-UCU_p5 AGTCCACTCAGCCATCTCTC AGAAGACCTATGTCCTATCCAT 55.0 40.9 58.5 55.2 1845-1880 bp 1.0 43.9