Hotspot

rpl16

LSC · 78599-80039 · 1441 bp

Back to Alloeochaete

Pi 0.00556

MarkerSeek score 50.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1441
variable_sites: 8
pi: 0.00556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CCAAAAAGAAAAACCCGGGG	TTTCCGAAATCCCTGAAAAA	50.0	35.0	57.8	53.3	1578 bp	0.5	20.1
rpl16_p2	CCAAAAAGAAAAACCCGGGG	TTTTCCGAAATCCCTGAAAA	50.0	35.0	57.8	53.3	1579 bp	0.5	20.1
rpl16_p3	CCAAAAAGAAAAACCCGGGG	TTTTTCCGAAATCCCTGAAA	50.0	35.0	57.8	53.3	1580 bp	0.5	20.1
rpl16_p4	CCAAAAAGAAAAACCCGGGG	TTTTCCGAAATCCCTGAAAAA	50.0	33.3	57.8	54.2	1579 bp	0.5	20.0
rpl16_p5	CCAAAAAGAAAAACCCGGGG	TTTTTCCGAAATCCCTGAAAA	50.0	33.3	57.8	54.2	1580 bp	0.5	20.0