Hotspot

ccsA

SSC · 107782-108744 · 963 bp

Back to Alloeochaete

Pi 0.00623
MarkerSeek score 49.80
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
963
variable_sites
6
pi
0.00623
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 TCCAATTTTGTAATGGGACCT GACGTTCAAGGGGTTCTCAA 38.1 50.0 55.1 57.8 1068-1074 bp 1.0 46.9
ccsA_p2 TCCAATTTTGTAATGGGACCTTC GACGTTCAAGGGGTTCTCAA 39.1 50.0 57.0 57.8 1068-1074 bp 1.0 46.5
ccsA_p3 CCAATTTTGTAATGGGACCTTCT GACGTTCAAGGGGTTCTCAA 39.1 50.0 57.0 57.8 1067-1073 bp 1.0 46.4
ccsA_p4 TCCAATTTTGTAATGGGACCTT GACGTTCAAGGGGTTCTCAA 36.4 50.0 55.8 57.8 1068-1074 bp 1.0 45.2
ccsA_p5 TTCCAATTTTGTAATGGGACCT GACGTTCAAGGGGTTCTCAA 36.4 50.0 55.8 57.8 1069-1075 bp 1.0 45.2