Hotspot

ndhH-rps15

IRa · 116433-116554 · 122 bp

Back to Alloeochaete

Pi 0.0164

MarkerSeek score 61.90

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: IRa
length_bp: 122
variable_sites: 2
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	AACACCATGCATTGATGGGT	GTTTGCTGGCTTATTTGGCA	45.0	45.0	58.1	57.5	277 bp	1.0	76.5
ndhH-rps15_p2	TCAGTCGAAGAACACCATGC	GTTTGCTGGCTTATTTGGCA	50.0	45.0	58.2	57.5	287 bp	1.0	75.3
ndhH-rps15_p3	ATGCATTGATGGGTGGTGAG	GTTTGCTGGCTTATTTGGCA	50.0	45.0	58.2	57.5	271 bp	1.0	75.1
ndhH-rps15_p4	AACACCATGCATTGATGGGT	CGTTTGCTGGCTTATTTGGC	45.0	50.0	58.1	58.7	278 bp	1.0	74.9
ndhH-rps15_p5	AACACCATGCATTGATGGGT	ACGTTTGCTGGCTTATTTGG	45.0	45.0	58.1	57.3	279 bp	1.0	74.3