Hotspot

rpl32-trnL-UAG

SSC · 107408-107624 · 217 bp

Back to Alloeochaete

Pi 0.0276
MarkerSeek score 62.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
217
variable_sites
6
pi
0.0276
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCAACGAGTATCCAAAACC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.1 57.9 298-301 bp 1.0 73.3
rpl32-trnL-UAG_p2 GGCAACGAGTATCCAAAACC AGCAGCGTGTCTACCAATTT 50.0 45.0 57.1 57.8 291-294 bp 1.0 72.9
rpl32-trnL-UAG_p3 GGCAACGAGTATCCAAAACC GCTTGAGCACTGCTTCCTAA 50.0 50.0 57.1 58.2 312-315 bp 1.0 72.7
rpl32-trnL-UAG_p4 GGCAACGAGTATCCAAAACC CCTAAGAGCAGCGTGTCTAC 50.0 55.0 57.1 57.8 297-300 bp 1.0 72.7
rpl32-trnL-UAG_p5 GGCAACGAGTATCCAAAACC CTAAGAGCAGCGTGTCTACC 50.0 55.0 57.1 57.8 296-299 bp 1.0 72.7