Hotspot

ndhF-rpl32

SSC · 106386-107239 · 854 bp

Back to Alloeochaete

Pi 0.00824
MarkerSeek score 62.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
854
variable_sites
7
pi
0.00824
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TGCCATCGAAGTACGTTTTT 55.0 40.0 58.1 56.0 937-983 bp 1.0 64.0
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TTGCCATCGAAGTACGTTTT 55.0 40.0 58.1 56.0 938-984 bp 1.0 64.0
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTTGCCATCGAAGTACGTTT 55.0 40.0 58.1 56.0 939-985 bp 1.0 64.0
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTTGCCATCGAAGTACGTT 55.0 40.0 58.1 56.0 940-986 bp 1.0 64.0
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TTTTTGCCATCGAAGTACGT 55.0 40.0 58.1 56.0 941-987 bp 1.0 64.0