Hotspot

rpl22-rps19

LSC · 81369-81456 · 88 bp

Back to Alloeochaete

Pi 0.0119

MarkerSeek score 63.70

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 88
variable_sites: 1
pi: 0.0119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TCTTGCGAGTTTCCTTAGCC	CAAATTGGGGGAATTCGTGC	50.0	50.0	57.9	58.0	238-242 bp	1.0	80.8
rpl22-rps19_p2	TCTTGCGAGTTTCCTTAGCC	GCAAATTGGGGGAATTCGTG	50.0	50.0	57.9	58.0	239-243 bp	1.0	80.8
rpl22-rps19_p3	CGGTTGGAACTTTTCTTGCG	CAAATTGGGGGAATTCGTGC	50.0	50.0	58.2	58.0	251-255 bp	1.0	79.5
rpl22-rps19_p4	CGGTTGGAACTTTTCTTGCG	GCAAATTGGGGGAATTCGTG	50.0	50.0	58.2	58.0	252-256 bp	1.0	79.5
rpl22-rps19_p5	TCTTGCGAGTTTCCTTAGCC	ATGGTAGGTCGCAAATTGGG	50.0	50.0	57.9	58.2	249-253 bp	1.0	78.9