Hotspot

trnW-CCA-trnP-UGG

LSC · 64809-64936 · 128 bp

Back to Alloeochaete

Pi 0.0159

MarkerSeek score 61.80

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 128
variable_sites: 2
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	ACCTACGACATCGGGTTTTG	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	240-242 bp	1.0	79.7
trnW-CCA-trnP-UGG_p2	ACCTACGACATCGGGTTTTG	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	239-241 bp	1.0	79.3
trnW-CCA-trnP-UGG_p3	TGGAATCACGCTCTGTAGGA	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	58.2	57.9	265-267 bp	1.0	79.1
trnW-CCA-trnP-UGG_p4	ACCTACGACATCGGGTTTTG	CGCGTTTGTTTTGGGTACAA	50.0	45.0	57.9	57.8	232-234 bp	1.0	79.1
trnW-CCA-trnP-UGG_p5	TGGAATCACGCTCTGTAGGA	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	58.2	58.2	264-266 bp	1.0	78.6