Hotspot

rbcL-psaI

LSC · 57170-58065 · 896 bp

Back to Alloeochaete

Pi 0.019

MarkerSeek score 69.70

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 896
variable_sites: 17
pi: 0.019
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CGATCAAATTCGAGTTCGCG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1007-1008 bp	1.0	81.0
rbcL-psaI_p2	CGATCAAATTCGAGTTCGCG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	1013-1014 bp	1.0	80.3
rbcL-psaI_p3	GCCGCAGCTTGTGAAATATG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	1032-1033 bp	1.0	79.8
rbcL-psaI_p4	GCCGCAGCTTGTGAAATATG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	1038-1039 bp	1.0	79.1
rbcL-psaI_p5	GATCAAATTCGAGTTCGCGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	1006-1007 bp	1.0	78.9