Hotspot

atpI-atpH

LSC · 32676-33482 · 807 bp

Back to Alloeochaete

Pi 0.0062

MarkerSeek score 62.40

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 807
variable_sites: 5
pi: 0.0062
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	ATTTTTGCAACGTTAGCCGC	GCAGTACCTTGACCAACTCC	45.0	55.0	58.6	58.2	955-956 bp	1.0	74.3
atpI-atpH_p2	ATTTTTGCAACGTTAGCCGC	AAGCAGCAGCAATTAGTGGA	45.0	45.0	58.6	57.8	888-889 bp	1.0	74.2
atpI-atpH_p3	ATTTTTGCAACGTTAGCCGC	GCCAATCCAGCAGCAATAAC	45.0	50.0	58.6	57.8	910-911 bp	1.0	74.1
atpI-atpH_p4	ATTTTTGCAACGTTAGCCGC	ATAACGGAAGCAGCAGCAAT	45.0	45.0	58.6	58.2	895-896 bp	1.0	73.9
atpI-atpH_p5	ATTTTTGCAACGTTAGCCGC	TCCAGCAGCAATAACGGAAG	45.0	50.0	58.6	58.3	905-906 bp	1.0	73.7