Hotspot

rpoB-rpoC1

LSC · 23851-23909 · 59 bp

Back to Alloeochaete

Pi 0.027

MarkerSeek score 62.50

Primer available yes

Label rpoB-rpoC1

Feature Metadata

label_name: rpoB-rpoC1
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 1
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.63
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-rpoC1_p1	CGAGGATCCTCCAGAATCCT	CTTCGCCAATAACTTCCCCA	55.0	50.0	58.0	57.9	264-286 bp	1.0	83.2
rpoB-rpoC1_p2	CGAGGATCCTCCAGAATCCT	ACTTCGCCAATAACTTCCCC	55.0	50.0	58.0	57.9	265-287 bp	1.0	83.2
rpoB-rpoC1_p3	CGAGGATCCTCCAGAATCCT	TTGTTGAGGGGAAACGAGTC	55.0	50.0	58.0	57.8	212-234 bp	1.0	82.3
rpoB-rpoC1_p4	ACGAGGATCCTCCAGAATCC	CTTCGCCAATAACTTCCCCA	55.0	50.0	58.3	57.9	265-287 bp	1.0	81.0
rpoB-rpoC1_p5	ACGAGGATCCTCCAGAATCC	ACTTCGCCAATAACTTCCCC	55.0	50.0	58.3	57.9	266-288 bp	1.0	81.0