Hotspot

trnD-GUC-psbM

LSC · 16408-17495 · 1088 bp

Back to Alloeochaete

Pi 0.00657

MarkerSeek score 64.60

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1088
variable_sites: 7
pi: 0.00657
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	TGGCTCGCGGTTTTTACATA	50.0	45.0	58.5	57.9	1224-1247 bp	1.0	77.4
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	TTTTGGCTCGCGGTTTTTAC	50.0	45.0	58.5	57.9	1227-1250 bp	1.0	77.1
trnD-GUC-psbM_p3	TTCAATTGGTCAGAGCACCG	TAATCATTTTGGCTCGCGGT	50.0	45.0	58.5	58.3	1233-1256 bp	1.0	76.1
trnD-GUC-psbM_p4	TAGGTATGCCATACACCCCG	TGGCTCGCGGTTTTTACATA	55.0	45.0	58.7	57.9	1256-1279 bp	1.0	75.9
trnD-GUC-psbM_p5	TAGGTATGCCATACACCCCG	TTTTGGCTCGCGGTTTTTAC	55.0	45.0	58.7	57.9	1259-1282 bp	1.0	75.7