Hotspot

trnG-UCC-trnT-GGU

LSC · 13890-15318 · 1429 bp

Back to Alloeochaete

Pi 0.00562
MarkerSeek score 55.00
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1429
variable_sites
8
pi
0.00562
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT ACCACTAAGTGAAAAGCCCT 38.1 45.0 55.5 56.7 1549-1572 bp 1.0 41.6
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 1519-1542 bp 1.0 41.4
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 1515-1538 bp 1.0 40.7
trnG-UCC-trnT-GGU_p4 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 1516-1539 bp 1.0 40.7
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT ACCACTAAGTGAAAAGCCCT 39.1 45.0 57.3 56.7 1551-1574 bp 1.0 40.7