Hotspot

trnS-GGA-psbD

LSC · 8136-9133 · 998 bp

Back to Alloeochaete

Pi 0.00409
MarkerSeek score 63.00
Primer available yes
Label trnS-GGA-psbD

Feature Metadata

label_name
trnS-GGA-psbD
feature_type
igs
region
LSC
length_bp
998
variable_sites
4
pi
0.00409
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-psbD_p1 ATTAGCAATCCGCCGCTTTA GCCGGACCATCCTACAAAAA 45.0 50.0 58.0 58.2 1125-1143 bp 1.0 80.0
trnS-GGA-psbD_p2 ATTAGCAATCCGCCGCTTTA ACAAAAACGAAACGGTCCCT 45.0 45.0 58.0 58.2 1112-1130 bp 1.0 79.4
trnS-GGA-psbD_p3 GCTTTAGTCCACTCAGCCAT GCCGGACCATCCTACAAAAA 50.0 50.0 57.6 58.2 1111-1129 bp 1.0 77.1
trnS-GGA-psbD_p4 ATTAGCAATCCGCCGCTTTA GAAACGGTCCCTTCGTAACC 45.0 55.0 58.0 58.6 1104-1122 bp 1.0 76.9
trnS-GGA-psbD_p5 GCTTTAGTCCACTCAGCCAT ACAAAAACGAAACGGTCCCT 50.0 45.0 57.6 58.2 1098-1116 bp 1.0 76.5