Hotspot

ycf1

SSC · 123006-128228 · 5223 bp

Pi 0.033

MarkerSeek score 67.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5223
variable_sites: 1968
pi: 0.033
unique_haplotype_count: 205
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCCCTTAACCACATTTTCCA	TCAGAGGCTTGGGATAGCTT	42.9	50.0	57.4	58.1	1392-1449 bp	0.823789	71.1
ycf1_p2	ACCCCTTAACCACATTTTCCA	ATCAGAGGCTTGGGATAGCT	42.9	50.0	57.4	57.9	1393-1450 bp	0.819383	70.9
ycf1_p3	ACCCCTTAACCACATTTTCCA	ATTGCAATTCCCCGAATGGT	42.9	45.0	57.4	58.1	1252-1318 bp	0.555066	60.1
ycf1_p4	ACCCCTTAACCACATTTTCCA	AATCATGGAGATCCGCGAAG	42.9	50.0	57.4	57.8	1101-1113 bp	0.422907	53.5
ycf1_p5	ACCCCTTAACCACATTTTCCA	AGATCCGCGAAGACGAAAAA	42.9	45.0	57.4	57.9	1093-1111 bp	0.387665	52.3