Hotspot

rps15-ycf1

SSC · 122644-123005 · 362 bp

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Pi 0.0572
MarkerSeek score 69.00
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
362
variable_sites
220
pi
0.0572
unique_haplotype_count
176
primer_available
yes
alignment_reliability
0.91
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AACACCATGCATTGATGGGT AATCTGTTGCACAACACAGC 45.0 45.0 58.1 57.5 1099-1225 bp 0.92511 84.6
rps15-ycf1_p2 CCACCGTGTTACATAAGGCA AATCTGTTGCACAACACAGC 50.0 45.0 57.9 57.5 1234-1360 bp 0.885463 82.6
rps15-ycf1_p3 TGACTCCCGAATACCCAACT CCCCAGACGTCGTAGAGAAT 50.0 55.0 58.1 58.6 826-940 bp 0.889868 82.3
rps15-ycf1_p4 TGACTCCCGAATACCCAACT AATCTGTTGCACAACACAGC 50.0 45.0 58.1 57.5 908-1025 bp 0.828194 80.7
rps15-ycf1_p5 TGACTCCCGAATACCCAACT ATCTGTTGCACAACACAGCA 50.0 45.0 58.1 58.6 907-1024 bp 0.828194 79.8