Hotspot

ndhF-rpl32

SSC · 111902-112283 · 382 bp

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Pi 0.056
MarkerSeek score 77.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
382
variable_sites
201
pi
0.056
unique_haplotype_count
188
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGAAAGGGTATTATCCATGC GCAACTAAATTTCCCTTTTTCTTCC 43.5 36.0 57.0 57.6 457-1134 bp 0.885463 47.4
ndhF-rpl32_p2 GGAAGAAAGGGTATTATCCATGC TGCAACTAAATTTCCCTTTTTCTTC 43.5 32.0 57.0 57.0 458-1135 bp 0.885463 47.4
ndhF-rpl32_p3 GGAAGAAAGGGTATTATCCATGC TGCAACTAAATTTCCCTTTTTCT 43.5 30.4 57.0 55.2 458-1135 bp 0.872247 46.9
ndhF-rpl32_p4 GGAAGAAAGGGTATTATCCATGC GCTTTTATTGCAACTAAATTTCCCT 43.5 32.0 57.0 57.1 466-1143 bp 0.76652 42.7
ndhF-rpl32_p5 AGGAAATGGAAGAAAGGGTA GCAACTAAATTTCCCTTTTTCTTCC 40.0 36.0 53.5 57.6 516-1043 bp 0.030837 11.4