Hotspot

psbE-petL

LSC · 64250-65236 · 987 bp

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Pi 0.0295
MarkerSeek score 76.40
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
987
variable_sites
393
pi
0.0295
unique_haplotype_count
180
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 ACGAATAACCAACCCGCAAT GGAGTTATAGTTGAAGCAGCCA 45.0 45.5 58.2 58.1 870-1181 bp 1.0 73.6
psbE-petL_p2 CAACCCGCAATGAATAGGGA GGAGTTATAGTTGAAGCAGCCA 50.0 45.5 57.9 58.1 861-1172 bp 0.973568 73.5
psbE-petL_p3 CAACCCGCAATGAATAGGGA TGGAGTTATAGTTGAAGCAGCC 50.0 45.5 57.9 58.1 862-1173 bp 0.973568 73.5
psbE-petL_p4 ACTGACGAATAACCAACCCG GGAGTTATAGTTGAAGCAGCCA 50.0 45.5 57.9 58.1 874-1185 bp 0.933921 71.6
psbE-petL_p5 ACTGACGAATAACCAACCCG TGGAGTTATAGTTGAAGCAGCC 50.0 45.5 57.9 58.1 875-1186 bp 0.933921 71.6