Hotspot

trnT_UGU-trnL_UAA

LSC · 46650-47672 · 1023 bp

Back to Allium

Pi 0.0405
MarkerSeek score 77.20
Primer available yes
Label trnT_UGU-trnL_UAA

Feature Metadata

label_name
trnT_UGU-trnL_UAA
feature_type
igs
region
LSC
length_bp
1023
variable_sites
522
pi
0.0405
unique_haplotype_count
188
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT_UGU-trnL_UAA_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 754-1387 bp 0.991189 90.5
trnT_UGU-trnL_UAA_p2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 762-1265 bp 0.982379 89.7
trnT_UGU-trnL_UAA_p3 GCGATGCTCTAACCTCTGAG ATCCAATCAAGTCCGTAGCG 55.0 50.0 57.9 57.8 777-1412 bp 1.0 89.1
trnT_UGU-trnL_UAA_p4 GCGATGCTCTAACCTCTGAG CAATCAAGTCCGTAGCGTCT 55.0 50.0 57.9 57.7 774-1409 bp 1.0 88.7
trnT_UGU-trnL_UAA_p5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 768-1271 bp 0.986784 88.5