Hotspot

psbM-trnD_GUC

LSC · 30222-31300 · 1079 bp

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Pi 0.0341
MarkerSeek score 79.80
Primer available yes
Label psbM-trnD_GUC

Feature Metadata

label_name
psbM-trnD_GUC
feature_type
igs
region
LSC
length_bp
1079
variable_sites
459
pi
0.0341
unique_haplotype_count
189
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD_GUC_p1 AGAATGAACAGTGCAGTAGC GTATGCTATACACCTCGCCG 45.0 55.0 55.8 58.0 902-1432 bp 0.881057 69.2
psbM-trnD_GUC_p2 AGTGCGAGAATATTGACTTCCA TTCAATTGGTCAGAGCACCG 40.9 50.0 57.5 58.5 803-1379 bp 1.0 68.4
psbM-trnD_GUC_p3 AGTGCGAGAATATTGACTTCCA GTATGCTATACACCTCGCCG 40.9 55.0 57.5 58.0 878-1408 bp 0.889868 67.6
psbM-trnD_GUC_p4 AGAATGAACAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 45.0 50.0 55.8 58.5 827-1403 bp 0.920705 67.2
psbM-trnD_GUC_p5 GTGCGAGAATATTGACTTCCA GTATGCTATACACCTCGCCG 42.9 55.0 56.1 58.0 877-1407 bp 0.889868 64.0