Hotspot

petN-psbM

LSC · 29269-30116 · 848 bp

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Pi 0.0358
MarkerSeek score 73.50
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
848
variable_sites
414
pi
0.0358
unique_haplotype_count
176
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 GTATGGGGAAGGAGTGGACT TGCTACTGCACTGTTCATTCT 55.0 42.9 58.1 57.6 694-1011 bp 0.898678 75.7
petN-psbM_p2 AGTATGGGGAAGGAGTGGAC TGCTACTGCACTGTTCATTCT 55.0 42.9 58.1 57.6 695-1012 bp 0.894273 75.6
petN-psbM_p3 TATGGGGAAGGAGTGGACTC TGCTACTGCACTGTTCATTCT 55.0 42.9 57.8 57.6 693-1010 bp 0.894273 75.1
petN-psbM_p4 GGGGAAGGAGTGGACTCTAG TGCTACTGCACTGTTCATTCT 60.0 42.9 58.2 57.6 690-1007 bp 0.894273 74.8
petN-psbM_p5 GTATGGGGAAGGAGTGGACT TGCTACTGCACTGTTCATTC 55.0 45.0 58.1 56.1 694-1011 bp 0.885463 71.0