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trnC_GCA-petN

LSC · 28189-29178 · 990 bp

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Pi 0.04
MarkerSeek score 76.40
Primer available yes
Label trnC_GCA-petN

Feature Metadata

label_name
trnC_GCA-petN
feature_type
igs
region
LSC
length_bp
990
variable_sites
477
pi
0.04
unique_haplotype_count
204
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC_GCA-petN_p1 CCAGTTCAAATCCGGGTGTC TTAAAGCAGCCCAAGCAAGA 55.0 45.0 58.8 58.0 642-1095 bp 0.982379 84.6
trnC_GCA-petN_p2 CCAGTTCAAATCCGGGTGTC CAGCCCAAGCAAGACTTACT 55.0 50.0 58.8 57.8 636-1089 bp 0.982379 83.1
trnC_GCA-petN_p3 CCAGTTCAAATCCGGGTGTC AGACGACCATTAAAGCAGCC 55.0 50.0 58.8 58.3 651-1104 bp 0.977974 82.4
trnC_GCA-petN_p4 TATCCCCAGTTCAAATCCGG TTAAAGCAGCCCAAGCAAGA 50.0 45.0 57.0 58.0 647-1100 bp 0.964758 82.4
trnC_GCA-petN_p5 CAGTTCAAATCCGGGTGTCG TTAAAGCAGCCCAAGCAAGA 55.0 45.0 59.2 58.0 641-1094 bp 0.982379 81.6