Hotspot

trnS_GCU-trnG_UCC

LSC · 8303-9480 · 1178 bp

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Pi 0.0464
MarkerSeek score 75.70
Primer available yes
Label trnS_GCU-trnG_UCC

Feature Metadata

label_name
trnS_GCU-trnG_UCC
feature_type
igs
region
LSC
length_bp
1178
variable_sites
621
pi
0.0464
unique_haplotype_count
191
primer_available
yes
alignment_reliability
0.94
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS_GCU-trnG_UCC_p1 GCTTTAGTCCACTCAGCCAT TGAACGAATCACACTTTTACCAC 50.0 39.1 57.6 57.6 754-1351 bp 1.0 61.4
trnS_GCU-trnG_UCC_p2 GCTTTAGTCCACTCAGCCAT ACGAATCACACTTTTACCACT 50.0 38.1 57.6 55.5 751-1348 bp 1.0 60.4
trnS_GCU-trnG_UCC_p3 AGTCCACTCAGCCATCTCTC TGAACGAATCACACTTTTACCAC 55.0 39.1 58.5 57.6 749-1346 bp 0.995595 60.4
trnS_GCU-trnG_UCC_p4 GCTTTAGTCCACTCAGCCAT GAACGAATCACACTTTTACCACT 50.0 39.1 57.6 57.3 753-1350 bp 1.0 59.4
trnS_GCU-trnG_UCC_p5 AGTCCACTCAGCCATCTCTC ACGAATCACACTTTTACCACT 55.0 38.1 58.5 55.5 746-1343 bp 0.995595 59.4